Double fluorescence technique for measurement of complement-fixing antibody to lymphocyte subsets. NLM AIDSLINE Important note: Information in this article was accurate in 1986. The state of the art may have changed since the publication date.

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Double fluorescence technique for measurement of complement-fixing antibody to lymphocyte subsets.

J Immunol Methods. 1985 Dec 17;85(1):163-8. Unique Identifier : AIDSLINE MED/86087268
Ozturk GE; Hayward AR; Weil R 3d; Kohler PF

Abstract: A double fluorescent antibody method for quantitating human complement-fixing antibody to lymphocyte subclasses has been developed. The indicators in this system are a C6-deficient serum as a non-lytic source of complement, rhodamine-labeled anti-C3 and fluorescein-labeled murine monoclonal antibodies to human lymphocyte subsets. The basic procedure is to incubate lymphocytes with the unknown serum and then to add C6-deficient serum. The binding of C3 is indicated by staining with rhodamine-labeled anti-C3 and the subset class of the lymphocyte so stained is determined by binding of fluorescein-tagged anti-OKT4 or -OKT8 antibodies. The occurrence of both red and green cell surface fluorescence denotes the presence of a complement-binding antibody to the lymphocyte subset defined by the monoclonal antibody. In addition to defining the specificity of complement-fixing anti-lymphocyte antibodies, this technique is more sensitive than the microcytotoxicity assay.
Keywords: Acquired Immunodeficiency Syndrome/IMMUNOLOGY Antibodies, Monoclonal/DIAGNOSTIC USE *Antibody-Dependent Cell Cytotoxicity Antigens, Surface/ANALYSIS Complement Fixation Tests *Fluorescent Antibody Technique Human Kidney/TRANSPLANTATION Kidney Transplantation Lupus Erythematosus, Systemic/IMMUNOLOGY Lymphocytes/*IMMUNOLOGY Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE

KWDacquiredimmunodeficiencysyndrome/immunologyantibodies,monoclonal/diagnosticuseKWDantibody-dependentcellcytotoxicityantigens,surface/analysiscomplementfixationtestsKWDfluorescentantibodytechniquehumankidney/transplantationkidneytransplantationlupuserythematosus,systemic/immunologylymphocytes/KWDimmunologysupport,uKWDsKWDgov't,pKWDhKWDsKWDjournalarticle
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M8640146

Copyright © 1986 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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