Purification and characterization of a human T-lymphocyte-derived glial growth-promoting factor.

Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.

Purification and characterization of a human T-lymphocyte-derived glial growth-promoting factor.

Proc Natl Acad Sci U S A. 1985 Jun;82(11):3930-4. Unique Identifier : AIDSLINE MED/85216607
Benveniste EN; Merrill JE; Kaufman SE; Golde DW; Gasson JC

Abstract: Antigen- or lectin-stimulated T lymphocytes and human T-cell leukemia virus (HTLV)-infected cell lines secrete lymphokines that can influence the growth and function of a variety of cell types. We recently demonstrated that supernatants from the HTLV-II-infected Mo-T-cell line stimulate the proliferation of rat brain oligodendrocytes and astrocytes. We have now purified a glial growth-promoting factor (GGPF) from these supernatants. Purification from serum-free conditioned medium was accomplished by sequential concentration, ammonium sulfate precipitation, lentillectin affinity chromatography, gel filtration, and reversed-phase high-performance liquid chromatography. GGPF is assayed by its ability to stimulate DNA synthesis in oligodendrocytes, as measured by [3H]thymidine uptake. The purified GGPF has an apparent Mr of 30,000 when analyzed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis under nonreducing conditions. Under reducing conditions, however, GGPF appears as a single band of Mr 18,000. Both reduced and unreduced forms have biological activity, suggesting that GGPF exists in both a functional monomeric and dimeric form. Purified GGPF appears to be a biochemically and functionally distinct lymphokine.

Keywords: Animal Cell Division/DRUG EFFECTS Cell Line Chromatography, Affinity Chromatography, Gel Chromatography, High Pressure Liquid Growth Substances/*ISOLATION & PURIF HTLV-BLV Viruses/*ANALYSIS *Lymphocyte Transformation Molecular Weight Nerve Tissue Proteins/*ISOLATION & PURIF/PHARMACOLOGY Oligodendroglia/DRUG EFFECTS Rats Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. T-Lymphocytes/*ANALYSIS JOURNAL ARTICLE

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