The location of cis-acting regulatory sequences in the human T cell lymphotropic virus type III (HTLV-III/LAV) long terminal repeat. NLM AIDSLINE Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.

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The location of cis-acting regulatory sequences in the human T cell lymphotropic virus type III (HTLV-III/LAV) long terminal repeat.

Cell. 1985 Jul;41(3):813-23. Unique Identifier : AIDSLINE GENBANK/K03455
Rosen CA; Sodroski JG; Haseltine WA

Abstract: The location of cis-acting regulatory sequences within the long terminal repeat (LTR) of the human T cell lymphotropic virus type III (HTLV-III/LAV) was determined. An enhancer element capable of increasing the rate of transcription from a heterologous promoter, irrespective of distance and orientation, is located between nucleotides -137 and -17 (cap site +1). The promoter sequences present near the TATA box respond to heterologous enhancers. The sequences present between nucleotides -17 and +80 are responsive to HTLV-III-associated trans-acting regulatory factors. Activation of these sequences by the viral regulatory factors requires the presence of a functional enhancer. The enhancer requirement is nonspecific, as the enhancer sequences of RSV, HTLV-I, and SV40 can functionally replace the HTLV-III enhancer. These findings define a new type of regulatory element, provide insight into the mechanisms that regulate HTLV-III gene expression, and may help to explain the effects of this virus on infected cells.
Keywords: Acetyltransferases/GENETICS Animal Cats Cell Line DNA, Recombinant *Enhancer Elements (Genetics) Gene Expression Regulation *Genes, Regulator Genes, Viral Genetic Complementation Test Human HTLV-BLV Viruses/*GENETICS Plasmids *Promoter Regions (Genetics) Repetitive Sequences, Nucleic Acid Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Transfection JOURNAL ARTICLE

KWDacetyltransferases/geneticsanimalcatscelllinedna,recombinantKWDenhancerelements(genetics)geneexpressionregulationKWDgenes,regulatorgenes,viralgeneticcomplementationtesthumanhtlv-blvviruses/KWDgeneticsplasmidsKWDpromoterregions(genetics)repetitivesequences,nucleicacidsupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDtransfectionjournalarticle
851030
M85A0078

Copyright © 1985 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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