Detection of HLA-D-region-associated antigens on the surface of adult T-cell leukemia virus particles by immunoelectron microscopy. NLM AIDSLINE Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.

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Detection of HLA-D-region-associated antigens on the surface of adult T-cell leukemia virus particles by immunoelectron microscopy.

Acta Med Okayama. 1984 Dec;38(6):505-10. Unique Identifier : AIDSLINE MED/85118524
Ohtsuki Y; Akagi T; Takeda I; Miyoshi I

Abstract: To search for lymphocyte marker antigens on the surface of human T-cell leukemia virus (HTLV), an immunoelectron microscopic study was performed on a HTLV-producing human T-cell line, MT-2, using monoclonal antibodies, such as anti-Leu-1, -Leu-2b, -Leu-3a, Leu-5, -Leu-10 and -HLA-DR and OKIal. The reactivity of each antibody with MT-2 cells was tested by the immunoperoxidase method at the light microscopic level. OKIal, anti-HLA-DR and Leu-10 gave positive results. At the ultrastructural level, the surface of HTLV as well as the plasma membranes of MT-2 cells were labeled with ferritin by the monoclonal antibodies OKIal, anti-HLA-DR and -Leu-10, but not by anti-Leu-1 and -Leu-3a. These findings suggest that HLA-D region -associated antigens are common antigenic determinants shared by the surface of HTLV and the plasma membranes of MT-2 cells. These antigens on the virus surface are probably picked up selectively from the plasma membranes and may play an important role in the interaction of HTLV and target T-cells.
Keywords: Antibodies, Monoclonal Histocompatibility Antigens Class II/*ANALYSIS Human HTLV-BLV Viruses/*IMMUNOLOGY Immunoenzyme Techniques Microscopy, Electron Support, Non-U.S. Gov't JOURNAL ARTICLE

KWDantibodies,monoclonalhistocompatibilityantigensclassii/KWDanalysishumanhtlv-blvviruses/KWDimmunologyimmunoenzymetechniquesmicroscopy,electronsupport,non-uKWDsKWDgov'tjournalarticle
850530
M8550083

Copyright © 1985 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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