Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.
Infectious transmission of human T-cell leukemia virus to rabbits.
Int J Cancer. 1985 Jan 15;35(1):81-5. Unique Identifier : AIDSLINE MED/85103945 Miyoshi I; Yoshimoto S; Kubonishi I; Fujishita M; Ohtsuki Y; Yamashita M; Yamato K; Hirose S; Taguchi H; Niiya K; et al
Abstract:
A rabbit lymphoid cell line (Ra-1) was established by co-cultivation with a human T-cell line (MT-2) carrying human T-cell leukemia virus (HTLV). The Ra-1 cell line is chromosomally male and is persistently infected with HTLV. Ra-1 cells, with or without mitomycin C treatment, were inoculated intravenously (i.v.) into 3 female rabbits. All 3 animals responded with the production of antibodies to HTLV antigens. Lymphocytes from one of these seroconverters were cultured in the presence of T-cell growth factor (TCGF) and HTLV particles were detected in the TCGF-grown lymphocytes which were chromosomally female. Co-cultivation of lymphocytes from the 2 other seroconverters with lymphocytes from 2 anti-HTLV-negative healthy men gave rise to the establishment of an HTLV-producing T-cell line derived from each individual. Blood transfusion from one of the HTLV-infected rabbits into 2 female rabbits also resulted in the seroconversion of both recipients. An HTLV-carrying lymphoid cell line (Ra-2) was established from one of the transfusion-related seroconverters. The Ra-2 cell line was initially TCGF-dependent but later became TCGF-independent. There results indicate that HTLV can be transmitted to rabbits. These animals may provide a suitable model system for studying the mode of transmission and pathogenicity of HTLV.
Keywords: Adult Animal Antibodies, Viral/ANALYSIS Blood Transfusion/ADVERSE EFFECTS Cell Line Cell Membrane/IMMUNOLOGY Female Human *HTLV-BLV Viruses/IMMUNOLOGY/ISOLATION & PURIF Karyotyping Leukemia/*MICROBIOLOGY Male Microscopy, Electron Middle Age Pregnancy Rabbits Retroviridae Infections/*TRANSMISSION Support, Non-U.S. Gov't T-Lymphocytes/CLASSIFICATION/MICROBIOLOGY/TRANSPLANTATION JOURNAL ARTICLE
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Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.
