Immunoassay for the detection and quantitation of infectious human retrovirus, lymphadenopathy-associated virus (LAV). NLM AIDSLINE Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.

Click here to return to AIDSLINE main menu
DonateNow
Print this Article


Immunoassay for the detection and quantitation of infectious human retrovirus, lymphadenopathy-associated virus (LAV).

J Immunol Methods. 1985 Jan 21;76(1):171-83. Unique Identifier : AIDSLINE MED/85106336
McDougal JS; Cort SP; Kennedy MS; Cabridilla CD; Feorino PM; Francis DP; Hicks D; Kalyanaraman VS; Martin LS

Abstract: Detection of replicating human retroviruses has relied upon rather cumbersome reverse transcriptase, immunofluorescence, or electron microscopic assays. We describe a new sandwich enzyme-linked immunoassay (ELISA) for detecting the human retrovirus, lymphadenopathy-associated virus (LAV), in supernates of LAV-infected human lymphocyte cultures. This LAV capture immunoassay compares favorably with the reverse transcriptase assay, despite the fact that it is performed on 20-fold less supernate material. Because the assay can be performed on 0.1 ml of culture supernate and is done by an ELISA method, LAV inoculation of lymphocyte cultures can be monitored quite conveniently, and endpoint titrations of infectious virus (ID-50 assays) can be performed. We demonstrate the application of the capture assay and ID-50 assay to disinfectant and serum neutralization experiments.
Keywords: Acquired Immunodeficiency Syndrome/MICROBIOLOGY Enzyme-Linked Immunosorbent Assay Human HTLV-BLV Viruses/*ANALYSIS Immunosorbent Techniques Lymphatic Diseases/MICROBIOLOGY Lymphocyte Transformation Methods JOURNAL ARTICLE

KWDacquiredimmunodeficiencysyndrome/microbiologyenzyme-linkedimmunosorbentassayhumanhtlv-blvviruses/KWDanalysisimmunosorbenttechniqueslymphaticdiseases/microbiologylymphocytetransformationmethodsjournalarticle
850530
M8550037

Copyright © 1985 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1985. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1985. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .