CHROMOSOMAL MAPPING OF TUMOR VIRUS TRANSFORMING GENE ANALOGS IN HUMAN CELLS NLM AIDSLINE Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.

Click here to return to AIDSLINE main menu
DonateNow
Print this Article


CHROMOSOMAL MAPPING OF TUMOR VIRUS TRANSFORMING GENE ANALOGS IN HUMAN CELLS

Prog Cancer Res Ther; 30:197-205, 1984. Unique Identifier : AIDSLINE ICDB/85605709
McBride OW; Swan DC; Robbins KC; Prakash K; Aaronson SA; Laboratory of Biochemistry, NCI, NIH, Bethesda, MD 20205

Abstract: A series of human/rodent hybrid somatic cell lines containing reduced complements of human chromosomes were analyzed as follows to determine the chromosomal location of cellular onc genes in human cells. The transforming gene (v-sis) of simian sarcoma virus (SSV) was subcloned in pBR322, and the human homolog (c-mos) of the transforming gene (v-mos) of Moloney murine sarcoma virus was cloned from a human recombinant phage library. Both the v-sis and the c-mos (human) were then used as molecular probes for detection of the corresponding cellular genes in EcoRI-digested, size-fractionated DNAs. By testing for the presence of v-sis and c-mos (human) in somatic cell hybrids possessing varying numbers of human chromosomes, it was possible to assign c-sis to chromosome 22 and c-mos (human) to chromosome 8. The location of these cellular onc genes was intriguing since very specific reciprocal translocations involving each of these chromosomes have previously been associated with specific human neoplasms. Neither the regional location of the onc genes relative to the specific chromosomal break points in the neoplastic cells nor the level of expression of these onc genes in the transformed cells has yet been determined.
Keywords: Animal Base Sequence Cell Line *Cell Transformation, Neoplastic *Cell Transformation, Viral *Chromosome Mapping Cloning, Molecular Cricetulus DNA Restriction Enzymes/GENETICS DNA, Recombinant Gene Expression Regulation Hamsters Human Hybrid Cells Leukemia/GENETICS Lymphoma/GENETICS Mice Nucleic Acid Hybridization *Oncogenes Sarcoma Viruses, Simian/GENETICS Translocation (Genetics) JOURNAL ARTICLE

KWDanimalbasesequencecelllineKWDcelltransformation,neoplasticKWDcelltransformation,viralKWDchromosomemappingcloning,molecularcricetulusdnarestrictionenzymes/geneticsdna,recombinantgeneexpressionregulationhamstershumanhybridcellsleukemia/geneticslymphoma/geneticsmicenucleicacidhybridizationKWDoncogenessarcomaviruses,simian/geneticstranslocation(genetics)journalarticle
850330
M8530162

Copyright © 1985 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1985. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1985. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .