Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.
Identification of an additional class of C3-binding membrane proteins of human peripheral blood leukocytes and cell lines.
Proc Natl Acad Sci U S A. 1985 Feb;82(3):859-63. Unique Identifier : AIDSLINE MED/85140195 Cole JL; Housley GA Jr; Dykman TR; MacDermott RP; Atkinson JP
Abstract:
Proteins binding the third component of complement (C3) were isolated by affinity chromatography from surface-labeled solubilized membranes of human peripheral blood cells and cell lines. The isolated molecules were subjected to NaDodSO4/PAGE, and autoradiographs of these gels indicated that C3-binding proteins could be divided into three groups based on Mr: (i) gp200, an approximately 200,000 Mr molecule previously identified as the C3b/C4b receptor or CR1; (ii) gp140, an approximately 140,000 Mr molecule previously identified as the C3d receptor or CR2; and (iii) gp45-70, a heretofore unrecognized group of 45,000-70,000 Mr C3-binding molecules. The cell distribution, Mr, antigenic cross-reactivity, and specificity of gp45-70 were examined. Erythrocytes have no detectable gp45-70, but all leukocyte populations examined possess this group of molecules. On neutrophils and mononuclear phagocytes, CR1 is the predominant C3-binding glycoprotein, but gp45-70 is present on both cell populations and on macrophage and neutrophil cell lines. B plus null cells, chronic lymphocytic leukemia cells, and an Epstein-Barr virus-transformed B-cell line possess CR1, CR2, and gp45-70. On T cells and T-cell lines gp45-70 is the predominant or, in some cases, the only C3-binding protein isolated. gp45-70 is structurally characterized as a broad band or doublet with a mean Mr that is slightly different for each cell population. gp45-70 binds iC3, C3b, and C4b, but not C3d, indicating that the binding region is probably within the C3c portion of C3b. A polyclonal antibody to CR1 and monoclonal antibodies to CR1 and CR2 do not immunoprecipitate gp45-70. While gp45-70 has not been previously characterized on human cells, a C3b-binding glycoprotein of similar Mr is present on rabbit alveolar macrophages. We conclude that gp45-70 is an additional group of membrane proteins present on human leukocytes that possess ligand-binding activity for C3b.
Keywords: B-Lymphocytes/ANALYSIS Carrier Proteins/*ANALYSIS Cell Line Chromatography, Affinity *Complement 3 Electrophoresis, Polyacrylamide Gel Human Leukemia, Lymphocytic/BLOOD Leukocytes/*ANALYSIS Membrane Proteins/*ANALYSIS Molecular Weight Monocytes/ANALYSIS Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. T-Lymphocytes/ANALYSIS JOURNAL ARTICLE
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Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.
