Human T-cell leukemia virus (HTLV-I) transcripts in fresh and cultured cells of patients with adult T-cell leukemia. NLM AIDSLINE Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.

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Human T-cell leukemia virus (HTLV-I) transcripts in fresh and cultured cells of patients with adult T-cell leukemia.

Proc Natl Acad Sci U S A. 1984 Oct;81(19):6207-11. Unique Identifier : AIDSLINE MED/85014935
Franchini G; Wong-Staal F; Gallo RC

Abstract: We examined the DNA and RNA of fresh leukemic cells (five patients) and long-term cultured T lymphocytes (four patients) from patients with adult T-cell leukemia for the presence of human T-cell leukemia virus (HTLV-I) sequences. In all cases HTLV-I provirus was found and several species of viral mRNAs were observed in all cell lines. We used HTLV DNA probes representing the gag, pol, and env genes and pX regions and the U3- and U5-specific sequences to characterize the genetic content of the viral transcripts. Although similar RNA sequences were expressed in one HTLV-I provirus-positive specimen of fresh primary leukemic cells, no viral transcripts were found in four other similar specimens. These findings are consistent with the idea that the expression of one or more HTLV-I genes may be involved in initiation of transformation, but consistent expression is not needed for maintaining the neoplastic state.
Keywords: Adult Cell Line Cells, Cultured DNA, Neoplasm/GENETICS/ISOLATION & PURIF DNA, Viral/GENETICS/ISOLATION & PURIF Human HTLV-BLV Viruses/*GENETICS Leukemia/*MICROBIOLOGY Nucleic Acid Hybridization Plasmids RNA, Neoplasm/GENETICS/ISOLATION & PURIF RNA, Viral/GENETICS/ISOLATION & PURIF T-Lymphocytes *Transcription, Genetic JOURNAL ARTICLE

KWDadultcelllinecells,cultureddna,neoplasm/genetics/isolation&purifdna,viral/genetics/isolation&purifhumanhtlv-blvviruses/KWDgeneticsleukemia/KWDmicrobiologynucleicacidhybridizationplasmidsrna,neoplasm/genetics/isolation&purifrna,viral/genetics/isolation&purift-lymphocytesKWDtranscription,geneticjournalarticle
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Copyright © 1985 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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