Fluorescence of Pneumocystis carinii in Papanicolaou smears. NLM AIDSLINE Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.

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Fluorescence of Pneumocystis carinii in Papanicolaou smears.

Hum Pathol. 1984 Oct;15(10):907-9. Unique Identifier : AIDSLINE MED/85005362
Ghali VS; Garcia RL; Skolom J


Abstract: Pneumocystis carinii organisms can be identified in routine cytologic smears stained by the Papanicolaou method and viewed under ultraviolet light in a fluorescence microscope. The organisms can be identified easily in thin, well-preserved cytologic smears. No false-positive results were obtained when 55 Papanicolaou smears of bronchial washings were observed by this method. Twenty-three of these smears were from patients with Pneumocystic carinii pneumonia in whom the acquired immunodeficiency syndrome (AIDS) had been well documented with Gomori's methenamine silver stain. With this method no additional special stains are required, no time delay for special stains is involved, and no additional material for the preparation of extra smears for special stains is needed. An additional advantage is the possibility of the retrospective analysis of smears already stained by the Papanicolaou method.
Keywords: Animal Bronchi/PARASITOLOGY False Negative Reactions Female Fluorescence Irrigation Microscopy, Fluorescence Pneumonia, Pneumocystis carinii/DIAGNOSIS/*PARASITOLOGY Vaginal Smears/*METHODS JOURNAL ARTICLE

KWDanimalbronchi/parasitologyfalsenegativereactionsfemalefluorescenceirrigationmicroscopy,fluorescencepneumonia,pneumocystiscarinii/diagnosis/KWDparasitologyvaginalsmears/KWDmethodsjournalarticle
850130
M8510008


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