A reliable silver staining method for identification of Pneumocystis carinii in histologic sections. NLM AIDSLINE Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.

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A reliable silver staining method for identification of Pneumocystis carinii in histologic sections.

Tohoku J Exp Med. 1984 Aug;143(4):397-404. Unique Identifier : AIDSLINE MED/85041218
Senba M

Abstract: The acquired immune deficiency syndrome (AIDS) is characterized by the development of Kaposi's sarcoma and several opportunistic infections including pneumonia caused by Pneumocystis carinii. As a staining method for Pneumocystis carinii, the methenamine-silver nitrate method has been used routinely. Most of fungi can be stained easily by this technique but the identification of Pneumocystis carinii is not always easy. The author has improved the method by using ammoniacal silver nitrate and found that this was a reliable staining method for Pneumocystis carinii. Moreover, this method proved to be superior to others in demonstrating Pneumocystis carinii in histologic sections.
Keywords: Aged Female Histocytochemistry Human Lung/PARASITOLOGY Male Middle Age Pneumocystis carinii/ANALYSIS Pneumonia, Pneumocystis carinii/*DIAGNOSIS/PARASITOLOGY Silver Stains and Staining/METHODS JOURNAL ARTICLE

KWDagedfemalehistocytochemistryhumanlung/parasitologymalemiddleagepneumocystiscarinii/analysispneumonia,pneumocystiscarinii/KWDdiagnosis/parasitologysilverstainsandstaining/methodsjournalarticle
850228
M8520085

Copyright © 1985 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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