Expression of the normal human sis/PDGF-2 coding sequence induces cellular transformation. NLM AIDSLINE Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.

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Expression of the normal human sis/PDGF-2 coding sequence induces cellular transformation.

Cell. 1984 Nov;39(1):89-97. Unique Identifier : AIDSLINE GENBANK/K01918
Gazit A; Igarashi H; Chiu IM; Srinivasan A; Yaniv A; Tronick SR; Robbins KC; Aaronson SA

Abstract: The human sis proto-oncogene contains the coding sequence for one of two polypeptide chains present in preparations of biologically active human platelet-derived growth factor (PDGF). A human clone, c-sis clone 8, which contains all of the v-sis-related sequences present in human DNA, was transcriptionally inactive when transfected into NIH/3T3 cells. When placed under the control of a retrovirus LTR, the clone was transcribed at levels comparable to that observed in cells transformed by SSV DNA. However, c-sis clone 8 DNA did not express detectable sis/PDGF-2 proteins and lacked biologic activity. A putative upstream exon was identified by its ability to detect the 4.2 kb sis-related transcript in certain human cells. When this sequence was inserted in the proper orientation between the LTR and c-sis clone 8, the chimeric molecule acquired high titered transforming activity, comparable to that of SSV DNA. Transformants containing this construct expressed human sis/PDGF-2 translational products. Thus the normal coding sequence for a human growth factor has transforming activity when expressed in an appropriate assay cell.
Keywords: Amino Acid Sequence Animal Base Sequence Cell Line *Cell Transformation, Neoplastic Cells, Cultured *Cloning, Molecular DNA Restriction Enzymes *Genes, Structural Glioma Human Mice Mice, Inbred Strains *Oncogenes Plasmids Platelet-Derived Growth Factor/*GENETICS Retroviridae/*GENETICS Sarcoma Viruses, Simian/*GENETICS Transfection JOURNAL ARTICLE

KWDaminoacidsequenceanimalbasesequencecelllineKWDcelltransformation,neoplasticcells,culturedKWDcloning,moleculardnarestrictionenzymesKWDgenes,structuralgliomahumanmicemice,inbredstrainsKWDoncogenesplasmidsplatelet-derivedgrowthfactor/KWDgeneticsretroviridae/KWDgeneticssarcomaviruses,simian/KWDgeneticstransfectionjournalarticle
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M8520054

Copyright © 1985 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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