HIGH LEVEL TRANSCRIPTION OF A HUMAN GENE IN HTLV POSITIVE T-CELLS: CDNA CLONING AND CHARACTERIZATION NLM AIDSLINE Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.

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HIGH LEVEL TRANSCRIPTION OF A HUMAN GENE IN HTLV POSITIVE T-CELLS: CDNA CLONING AND CHARACTERIZATION

Genetic and Phenotypic Markers of Tumors. Aaronson SA, Frati L, Verna R, eds. New York, Plenum Press, p. 345-55, 1984.. Unique Identifier : AIDSLINE ICDB/85615483
Fazio VM; Manzari V; Frati L; Franchini G; Wong-Staal F; Gallo RC; Istituto di Patologia Generale, Policlinico Umberto I, Universita; degli Studi di Roma, Italy

Abstract: The transcription of human T lymphocytes infected by human T-cell leukemia/lymphoma virus (HTLV) was analyzed to investigate the influence of HTLV integration in neoplastic transformation and proliferation of T-cells and to detect gene(s) expressed at high levels in these cells. Messenger RNA (mRNA) was extracted and selected from a T-cell line (HUT 102) actively producing HTLV; using this mRNA as a template, a complementary DNA (cDNA) library was constructed and a clone (HT-3) was selected that hybridized specifically with HUT 102 cDNA and not HUT 78. For selection of the HT-3 clone, the cDNA library was analyzed by differential screening with two different probes; homologous radiolabeled cDNA and labeled cDNA obtained from mRNA of HUT 78. This HT-3 clone hybridized specifically to a cellular single species mRNA of approx 2.3 kilobase, produced at detectable levels in lectin-activated T-cells and at very high levels only in HTLV-infected T-cells. Although no certain candidate can be indicated to HT-3 gene product identification, HT-3 may be involved in at least one of the steps related to T-cell growth factor production. The findings suggested that HT-3 gene is a cellular gene, not a proviral sequence, and that it is present in all human DNAs as a single copy per haploid genome, with at least two allelic genotypes among individuals. The correlation between HTLV infection and HT-3 high levels of transcription needs to be investigated. (30 Refs)
Keywords: Cell Line Cell Transformation, Neoplastic *Cloning, Molecular Gene Expression Regulation Human HTLV-BLV Viruses/*GENETICS Interleukin-2/GENETICS Oncogenes T-Lymphocytes/MICROBIOLOGY *Transcription, Genetic MEETING PAPER

KWDcelllinecelltransformation,neoplasticKWDcloning,moleculargeneexpressionregulationhumanhtlv-blvviruses/KWDgeneticsinterleukin-2/geneticsoncogenest-lymphocytes/microbiologyKWDtranscription,geneticmeetingpaper
851230
M85C0210

Copyright © 1985 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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