Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.
IMPAIRED INTERLEUKIN-2 PRODUCTION IN RESPONSE TO PHA AND OKT3 ANTIBODY IN MALE HOMOSEXUALS WITH ACQUIRED IMMUNE DEFICIENCY SYNDROME: IN VITRO RESTORATION OF T-CELL PROLIFERATION BY HIGHLY PURIFIED INTERLEUKIN-2
AIDS. The Epidemic of Kaposi's Sarcoma and Opportunistic Infections. Friedman-Kien AE, Laubenstein LJ, eds. New York, Masson, p. 199-205, 1984.. Unique Identifier : AIDSLINE ICDB/85610702 Welte K; Ciobanu N; Kruger G; Venuta S; Feldman SP; Sternberg C; Palladino MA; Koziner B; Gold J; Safai B; et al; Dept. of Hematology, Memorial Sloan-Kettering Cancer Center, New; York, NY 10021
Abstract:
To elucidate the decreased responsiveness to mitogen stimulation in patients (pts) with the Acquired Immune Deficiency Syndrome (AIDS), peripheral blood lymphocytes (PBLs) from 21 homosexual males were studied for response to OKT3 antibody and to phytohemagglutinin (PHA); for production of interleukin-2 (IL-2), a mediator of T-cell mitogen stimulation; and for the possibility of restoring the proliferative response by adding highly purified IL-2. Twelve of the patients had Kaposi's sarcoma (KS), 4 had generalized reactive lymphadenopathy (RL), and 5 had recurrent opportunistic infections (OIs). PBLs from KS and OI pts produced significantly lower amounts of IL-2 than age-matched heterosexual controls in response to stimulation by the OKT3 antibody or the PHA. DNA synthesis in PBLs from KS and OI pts, as measured by thymidine incorporation following OKT3 antibody or PHA stimulation, was significantly decreased compared with controls. Pts with RL had decreased DNA synthesis in response to the OKT3 antibody but not to the PHA. All 10 pts with thymidine incorporation greater than 13,000 counts/min were alive at 8 mo, while 5 of 11 pts with low incorporation died. When purified IL-2, was added to PBL cultures that were stimulated by OKT3 antibody, thymidine incorporation was increased to the normal range in 7 of the 12 pts with KS. The authors conclude that PBLs from the homosexual pts failed to proliferate normally when challenged by OKT3 antibody and that this abnormality was corrected in some pts by the administration of IL-2. They propose that T-cells from these pts have decreased production of, or sensitivity to, IL-2 and that in vivo administration of IL-2 may be of benefit in the treatment of AIDS. (26 Refs)
Keywords: Acquired Immunodeficiency Syndrome/COMPLICATIONS/*IMMUNOLOGY Adult Antibodies, Monoclonal/IMMUNOLOGY Human Interleukin-2/*BIOSYNTHESIS/IMMUNOLOGY/ISOLATION & PURIF Lymphocyte Transformation Male Middle Age Phytohemagglutinins Sarcoma, Kaposi's/COMPLICATIONS T-Lymphocytes/*IMMUNOLOGY MEETING PAPER
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Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.
