Molecular cloning of AIDS-associated retrovirus. NLM AIDSLINE Important note: Information in this article was accurate in 1985. The state of the art may have changed since the publication date.

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Molecular cloning of AIDS-associated retrovirus.

Nature. 1984 Dec 20-1985 Jan 2;312(5996):760-3. Unique Identifier : AIDSLINE MED/85086250
Luciw PA; Potter SJ; Steimer K; Dina D; Levy JA

Abstract: Retroviruses cause a wide variety of diseases in avian and mammalian species. Human acquired immune deficiency syndrome (AIDS) leads to collapse of the immune system and death by a wide variety of opportunistic infections; unusual forms of cancer are associated with this syndrome. Retroviruses have been recovered from tissues of AIDS patients and from patients with related conditions. These similar newly-isolated viruses are lymphadenopathy-associated virus (LAV), human T-cell lymphotropic virus (HTLV-III) and AIDS-associated retrovirus (ARV-2). We have identified a RNA genome of approximately 9 kilobases (kb) in virions purified from the culture medium of a human T-cell tumour line infected with ARV-2. A cDNA probe made from viral RNA detected circular DNA molecules and proviral forms in infected cells. We prepared a library of infected cell DNA. Recombinant phage included those with a 9.5-kb proviral DNA and viral DNA permuted with respect to the single EcoRI site. Comparison of three ARV isolates from different AIDS patients revealed polymorphism of restriction endonuclease sites.
Keywords: Acquired Immunodeficiency Syndrome/*MICROBIOLOGY Cloning, Molecular DNA Restriction Enzymes/DIAGNOSTIC USE DNA, Viral/GENETICS Human Polymorphism (Genetics) Retroviridae/*GENETICS/ISOLATION & PURIF Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE

KWDacquiredimmunodeficiencysyndrome/KWDmicrobiologycloning,moleculardnarestrictionenzymes/diagnosticusedna,viral/geneticshumanpolymorphism(genetics)retroviridae/KWDgenetics/isolation&purifsupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDjournalarticle
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Copyright © 1985 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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