Adenovirus isolates from urine of patients with acquired immunodeficiency syndrome. NLM AIDSLINE Important note: Information in this article was accurate in 1983. The state of the art may have changed since the publication date.

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Adenovirus isolates from urine of patients with acquired immunodeficiency syndrome.

Lancet. 1983 Jun 11;1(8337):1293-6. Unique Identifier : AIDSLINE MED/83217893
de Jong PJ; Valderrama G; Spigland I; Horwitz MS

Abstract: 13 adenoviruses (Ad) from the urine of 10 patients with acquired immunodeficiency syndrome (AIDS) were characterised by haemagglutination inhibition and restriction endonuclease analysis of their DNA. The haemagglutinin (HA) for 6 of these isolates was found to be that of Ad34/35. 3 other isolates were found to have Ad7 HA and the remaining 4 viruses were found to have both phenotypes. In contrast, the restriction patterns were more homogeneous than anticipated from the serological analysis. 11 isolates had a SmaI-restriction pattern identical to Ad35, and 2 isolates, which lacked one of the 9 Ad35 SmaI-restriction sites, were identical to Ad34. Analyses with other restriction enzymes reinforced the conclusion that the genomes of all isolates resemble that of Ad35 (and Ad34) more than they resembled the previously isolated Ad7 subtypes. The discrepancy between the restriction endonuclease and the serological analyses is best explained by assuming that some of these new isolates are recombinants between a small part (less than 10%) of the Ad7 genome coding for HA and greater than 90% of the Ad35 genome. It is therefore important to characterise both the genotype and the HA for this potentially important group of pathogens in AIDS patients.
Keywords: Acquired Immunodeficiency Syndrome/*MICROBIOLOGY Adenoviridae/CLASSIFICATION/GENETICS/*ISOLATION & PURIF DNA Restriction Enzymes/METABOLISM Female Genotype Hemagglutination Inhibition Tests Hemagglutination, Viral Hemagglutinins, Viral/GENETICS Human Male Phenotype Recombination, Genetic Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Urine/*MICROBIOLOGY JOURNAL ARTICLE

KWDacquiredimmunodeficiencysyndrome/KWDmicrobiologyadenoviridae/classification/genetics/KWDisolation&purifdnarestrictionenzymes/metabolismfemalegenotypehemagglutinationinhibitiontestshemagglutination,viralhemagglutinins,viral/geneticshumanmalephenotyperecombination,geneticsupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDurine/KWDmicrobiologyjournalarticle
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M8390004

Copyright © 1983 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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