Molecular cloning of circular unintegrated DNA of two types of the SEATO strain of gibbon ape leukemia virus. NLM AIDSLINE Important note: Information in this article was accurate in 1983. The state of the art may have changed since the publication date.

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Molecular cloning of circular unintegrated DNA of two types of the SEATO strain of gibbon ape leukemia virus.

J Virol. 1982 Oct;44(1):269-75. Unique Identifier : AIDSLINE MED/83059877
Gelmann EP; Trainor CD; Wong-Staal F; Reitz MS

Abstract: Closed circular unintegrated DNA of the SEATO strain of gibbon ape leukemia virus (GaLV-S) was isolated from canine thymus fibroblasts after cocultivation with chronically infected bat lung fibroblasts. Restriction endonuclease HindIII cleaves GaLV-S DNA once, thus allowing isolation and cloning of HindIII-digested unintegrated DNA in a permitted form. Two clones isolated in the vector, Charon 21A, were nearly identical by restriction enzyme mapping to each of the two types of GaLV-S previously observed. These two types differ at a single SalI site. Unlike previous maps of GaLV-S proviral DNA, however, both clones lack SstI sites in the long-terminal-repeat units. Both the GaLV-S clones and the major species of GaLV-S proviral DNA contain an EcoRI site in the long-terminal-repeat units. The presence of this EcoRI site and the absence of an SstI site in the GaLV-S long-terminal-repeat units differentiate it from all other known GaLV strains and from the closely related nononcogenic simian sarcoma-associated virus. Heteroduplex comparisons of each of the two clones to clones of simian sarcoma-associated virus show no obvious deletion or substitution loops. This suggests that the ability of GaLV-S to induce myeloid leukemia in gibbon apes in not due to an acquired onc gene.
Keywords: Animal Base Sequence *Cloning, Molecular Comparative Study DNA Restriction Enzymes DNA, Circular/*GENETICS DNA, Recombinant DNA, Viral/*GENETICS *Genes, Viral Helper Viruses/GENETICS Hylobates Nucleic Acid Heteroduplexes Repetitive Sequences, Nucleic Acid Retroviridae/*GENETICS Sarcoma Viruses, Simian/GENETICS JOURNAL ARTICLE

KWDanimalbasesequenceKWDcloning,molecularcomparativestudydnarestrictionenzymesdna,circular/KWDgeneticsdna,recombinantdna,viral/KWDgeneticsKWDgenes,viralhelperviruses/geneticshylobatesnucleicacidheteroduplexesrepetitivesequences,nucleicacidretroviridae/KWDgeneticssarcomaviruses,simian/geneticsjournalarticle
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Copyright © 1983 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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