Nucleotide sequence of the simian sarcoma virus genome: demonstration that its acquired cellular sequences encode the transforming gene product p28sis. NLM AIDSLINE Important note: Information in this article was accurate in 1983. The state of the art may have changed since the publication date.

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Nucleotide sequence of the simian sarcoma virus genome: demonstration that its acquired cellular sequences encode the transforming gene product p28sis.

Proc Natl Acad Sci U S A. 1983 Feb;80(3):731-5. Unique Identifier : AIDSLINE GENBANK/J02394
Devare SG; Reddy EP; Law JD; Robbins KC; Aaronson SA

Abstract: The complete nucleotide sequence of the proviral genome of simian sarcoma virus (SSV), an acute transforming retrovirus of primate origin, has been determined. Like other transforming viruses, SSV contains sequences derived from its helper virus, simian sarcoma-associated virus (SSAV), and a cell-derived (v-sis) insertion sequence. By comparison with the sequence of Moloney murine leukemia virus, it was possible to precisely localize and define sequences contributed by SSAV during the generation of SSV. Comparative sequence analysis of SSV and SSAV showed that SSAV provides regulatory sequences for initiation and termination of transcription of the SSV transforming gene. Moreover, coding sequences for the putative protein product of this gene appear to initiate from the amino terminus of the SSAV env gene. Antibodies to synthetic peptides derived from the carboxy and amino termini of the putative protein predicted by the open reading frame identified within v-sis specifically detect a Mr 28,000 protein, p28sis, in SSV-transformed cells. These and other findings confirm the predicted amino acid sequence of this protein and localize it to the coding region of the SSV transforming gene.
Keywords: Amino Acid Sequence Base Sequence *Cell Transformation, Viral Genes, Structural *Genes, Viral *Oncogenes Retroviridae/*GENETICS Sarcoma Viruses, Simian/*GENETICS Transcription, Genetic Translation, Genetic Viral Proteins/GENETICS JOURNAL ARTICLE

KWDaminoacidsequencebasesequenceKWDcelltransformation,viralgenes,structuralKWDgenes,viralKWDoncogenesretroviridae/KWDgeneticssarcomaviruses,simian/KWDgeneticstranscription,genetictranslation,geneticviralproteins/geneticsjournalarticle
830630
M8360012

Copyright © 1983 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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