Search for proviral simian sarcoma associated virus sequences in bone marrow cells from children with neoplasms of mesenchymal origin. NLM AIDSLINE Important note: Information in this article was accurate in 1983. The state of the art may have changed since the publication date.

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Search for proviral simian sarcoma associated virus sequences in bone marrow cells from children with neoplasms of mesenchymal origin.

Leuk Res. 1983;7(3):323-9. Unique Identifier : AIDSLINE MED/83295989
Jore JP; Dubbes RH

Abstract: DNAs obtained from bone marrow cells of 31 children with neoplasms of mesenchymal origin were tested for the presence of proviral simian sarcoma associated virus by southern blot-hybridization. The lower limit of detection in this method was one provirus per 20-30 cells. Applying stringent conditions of hybridization, no proviral DNA could be detected in any of the samples tested, most of which were from children with leukemia or with bone marrow invading lymphosarcomas. Relaxed conditions of hybridization revealed a diffuse pattern of hybridizing fragments which was similar in all DNAs tested, including normal human DNA.
Keywords: Bone Marrow/*MICROBIOLOGY Cell Line Child DNA, Viral/GENETICS/*ISOLATION & PURIF Human Leukemia/*MICROBIOLOGY Molecular Weight Nucleic Acid Hybridization Retroviridae/*GENETICS Rhabdomyosarcoma/MICROBIOLOGY Sarcoma/*MICROBIOLOGY Sarcoma Viruses, Simian/*GENETICS Support, Non-U.S. Gov't JOURNAL ARTICLE

KWDbonemarrow/KWDmicrobiologycelllinechilddna,viral/genetics/KWDisolation&purifhumanleukemia/KWDmicrobiologymolecularweightnucleicacidhybridizationretroviridae/KWDgeneticsrhabdomyosarcoma/microbiologysarcoma/KWDmicrobiologysarcomaviruses,simian/KWDgeneticssupport,non-uKWDsKWDgov'tjournalarticle
831230
M83C0021

Copyright © 1983 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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