Important note: Information in this article was accurate in 1983. The state of the art may have changed since the publication date.
Three-step stool examination for cryptosporidiosis in 10 homosexual men with protracted watery diarrhea.
J Infect Dis. 1983 May;147(5):824-8. Unique Identifier : AIDSLINE MED/83187717 Ma P; Soave R
Abstract:
Cryptosporidiosis, a zoonosis caused by Cryptosporidium species, is a newly recognized coccidial protozoan infection causing severe protracted watery diarrhea in humans. In August 1981, the first case of cryptosporidiosis in a homosexual man with acquired immune deficiency syndrome (AIDS) was reported; diagnosis was determined by intestinal biopsy. It is necessary to adopt a simple laboratory diagnostic procedure to screen large numbers of suspected cases. A three-step stool examination was developed to demonstrate Cryptosporidium oocysts and the diagnostic and infective stages of the infection in 10 homosexual men with AIDS. This is a less invasive, less costly, and more sensitive test than intestinal biopsy and has been designed to prevent confusion caused by yeast cells that are frequently present in stool, leading to a false diagnosis. The examination consists of preliminary differential determination by iodine wet mount, definitive identification by modified Kinyoun acid-fast staining, and a more effective method of concentrating oocysts, by Sheather's sugar cover-slip flotation method.
Keywords: Acquired Immunodeficiency Syndrome/COMPLICATIONS Coccidiosis/*PARASITOLOGY Diarrhea/*PARASITOLOGY Feces/*PARASITOLOGY *Homosexuality Human Male Support, Non-U.S. Gov't JOURNAL ARTICLE
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