Nucleotide sequence of the transforming gene of simian sarcoma virus. NLM AIDSLINE Important note: Information in this article was accurate in 1982. The state of the art may have changed since the publication date.

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Nucleotide sequence of the transforming gene of simian sarcoma virus.

Proc Natl Acad Sci U S A. 1982 May;79(10):3179-82. Unique Identifier : AIDSLINE GENBANK/J02397
Devare SG; Reddy EP; Robbins KC; Andersen PR; Tronick SR; Aaronson SA

Abstract: The sequence of the transforming region of simian sarcoma virus (SSV) has been determined by using molecularly cloned viral DNA. This region encompassed the 1.0-kilobase pair woolly monkey cell-derived insertion sequence, v-sis, and flanking simian sarcoma-associated viral (SSAV) sequences. A 675-nucleotide-long open reading frame commenced 19 nucleotides within the SSAV sequences to the left of the v-sis helper viral junction and terminated within v-sis itself. Possible promoter and acceptor splice signals were detected in helper viral sequences upstream from this open reading frame, and potential polyadenylylation sites were identified downstream both within v-sis and in helper viral sequences beyond v-sis. The recombinational event that led to the generation of SSV occurred in the middle of two functional codons, indicating that SSAV provided the regulatory elements for transcription as well as the initiation codon for translation of SSV cell-derived transforming sequences.
Keywords: *Cell Transformation, Viral DNA, Viral/GENETICS Gene Expression Regulation Genes, Regulator Genes, Structural *Genes, Viral Helper Viruses/GENETICS Retroviridae/*GENETICS RNA, Viral Sarcoma Viruses, Simian/*GENETICS Transcription, Genetic Viral Proteins/GENETICS JOURNAL ARTICLE

KWDcelltransformation,viraldna,viral/geneticsgeneexpressionregulationgenes,regulatorgenes,structuralKWDgenes,viralhelperviruses/geneticsretroviridae/KWDgeneticsrna,viralsarcomaviruses,simian/KWDgeneticstranscription,geneticviralproteins/geneticsjournalarticle
821130
M82B0003

Copyright © 1982 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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