USE OF SEPHAROSE BEAD IMMUNOFLUORESCENCE ASSAY FOR COMPARISON OF THE TYPE D RETROVIRUSES MPMV AND PMFV NLM AIDSLINE Important note: Information in this article was accurate in 1982. The state of the art may have changed since the publication date.

Click here to return to AIDSLINE main menu
DonateNow
Print this Article


USE OF SEPHAROSE BEAD IMMUNOFLUORESCENCE ASSAY FOR COMPARISON OF THE TYPE D RETROVIRUSES MPMV AND PMFV

Arch Geschwulstforsch; 52(4):275-282 1982. Unique Identifier : AIDSLINE ICDB/82617677
Micheel B; Uckert W; Denner J; Fiebach H; Wunderlich V; Dept. Experimental Clinical Immunology, Central Inst. Cancer; Res., Acad. Sciences GDR, Lindenberger Weg 80, DDR-1115; Berlin-Buch, E. Germany

Abstract: Experiments using the Sepharose bead immunofluorescence assay (SBIFA) for comparing Mason-Pfizer monkey virus (MPMV) and a type D retrovirus isolated from a continuous human fibroblast line (PMFV) are reported. MPMV p27 related antigens were demonstrated in experiments using sandwich SBIFA and in those using competition SBIFA. The p15 of PMFV contained antigenic determinants not present in MPMV. Both viruses reacted almost identically in the PMFV p27-specific assay, while there was no reaction of MPMV and a distinct reaction of PMFV in the PMFV p15-specific assay. (19 Refs)
Keywords: Animal Carcinoma, Squamous Cell/MICROBIOLOGY Cell Line Dogs Female Fluorescent Antibody Technique Human In Vitro Macaca mulatta Mammary Neoplasms, Experimental/MICROBIOLOGY Methods Ovarian Neoplasms/MICROBIOLOGY Retroviridae/*ANALYSIS Rhabdomyosarcoma/MICROBIOLOGY Sarcoma Viruses, Simian/ANALYSIS Sepharose Species Specificity Thymus Gland/MICROBIOLOGY Viral Proteins/ISOLATION & PURIF JOURNAL ARTICLE

KWDanimalcarcinoma,squamouscell/microbiologycelllinedogsfemalefluorescentantibodytechniquehumaninvitromacacamulattamammaryneoplasms,experimental/microbiologymethodsovarianneoplasms/microbiologyretroviridae/KWDanalysisrhabdomyosarcoma/microbiologysarcomaviruses,simian/analysissepharosespeciesspecificitythymusgland/microbiologyviralproteins/isolation&purifjournalarticle
820830
M8280015

Copyright © 1982 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1982. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1982. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .