Studies on Proteins of simian sarcoma-associated virus with different growth history. NLM AIDSLINE Important note: Information in this article was accurate in 1982. The state of the art may have changed since the publication date.

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Studies on Proteins of simian sarcoma-associated virus with different growth history.

J Gen Virol. 1982 Mar;59(Pt 1):65-71. Unique Identifier : AIDSLINE MED/82169405
Warnaar SO; Huiskens-Van der Meij C; Kool-Kofoed L; Mooren HW

Abstract: Simian sarcoma-associated virus (SSaV) was repeatedly passaged on three human cell lines. The proteins of the progeny virus were analysed for the presence of variant polypeptides. Occasionally, a few variant polypeptides were observed. One-dimensional peptide maps of the major virus protein p30 revealed no modifications after 25 cycles of infection on the three cell lines studied. The peptide map of Pr65gag of virus grown through 25 passages on a human chondrosarcoma cell line was slightly different from that of the virus stock before passaging. The relative amount of the virus protein p30 as compared to p18 and p16 (possibly the SSaV equivalents of p15E and p12E) was variable depending on the host cell. Virus grown on Daudi cells was relatively deficient in p18 and p16. These virus particles were morphologically altered and had a low infectivity.
Keywords: Animal Burkitt's Lymphoma Cell Line Chondrosarcoma Comparative Study Dogs Electrophoresis, Polyacrylamide Gel Human Peptides/ANALYSIS Retroviridae/*ANALYSIS Rhabdomyosarcoma Sarcoma Viruses, Simian/*ANALYSIS/GROWTH & DEVELOPMENT/ ULTRASTRUCTURE Support, Non-U.S. Gov't Thymus Gland Viral Proteins/*ANALYSIS JOURNAL ARTICLE

KWDanimalburkitt'slymphomacelllinechondrosarcomacomparativestudydogselectrophoresis,polyacrylamidegelhumanpeptides/analysisretroviridae/KWDanalysisrhabdomyosarcomasarcomaviruses,simian/KWDanalysis/growth&development/ultrastructuresupport,non-uKWDsKWDgov'tthymusglandviralproteins/KWDanalysisjournalarticle
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M8280005

Copyright © 1982 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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