Molecular cloning of integrated simian sarcoma virus: genome organization of infectious DNA clones. NLM AIDSLINE Important note: Information in this article was accurate in 1981. The state of the art may have changed since the publication date.

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Molecular cloning of integrated simian sarcoma virus: genome organization of infectious DNA clones.

Proc Natl Acad Sci U S A. 1981 May;78(5):2918-22. Unique Identifier : AIDSLINE MED/81247399
Robbins KC; Devare SG; Aaronson SA

Abstract: The integrated form of simian sarcoma virus (SSV) was molecularly cloned in the Charon 16A strain of bacteriophage lambda. In transfection analysis, the recombinant viral DNAs demonstrated the ability to transform cells in tissue culture at high efficiency. Such transformants possessed typical SSV morphology, expressed simian sarcoma associated virus (SSAV) gag gene products in the absence of virus release, and released SSV after superinfection with a type C helper virus. A physical map of the 5.8-kilobase-pair (kbp) recombinant viral DNA clone, deduced from restriction endonuclease analysis, revealed a 5.1-kbp SSV genome containing 0.55-kbp-long terminal repeats flanked by 0.45 and 0.25 kbp of contiguous host cell sequences. By R-loop analysis, the viral DNA molecule contained two regions of homology to SSAV, separated by a 1.0-kbp nonhomologous region. This SSV-specific sequence was shown to be uniquely represented within the normal cellular DNA of diverse mammalian species, including human. Our results demonstrate that this primate transforming retrovirus arose in nature by recombination of a type C helper virus and a host cellular gene.
Keywords: Animal Base Sequence Cell Line *Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/*METABOLISM DNA, Viral/*GENETICS *Genes, Viral Human Microscopy, Electron Nucleic Acid Conformation Retroviridae/*GENETICS Sarcoma Viruses, Simian/*GENETICS Transfection JOURNAL ARTICLEKWDanimalbasesequencecelllineKWDcloning,moleculardnarestrictionenzymesdna,recombinant/KWDmetabolismdna,viral/KWDgeneticsKWDgenes,viralhumanmicroscopy,electronnucleicacidconformationretroviridae/KWDgeneticssarcomaviruses,simian/KWDgeneticstransfectionjournalarticle
811130
M81B0003

Copyright © 1981 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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