Molecular cloning and comparative analyses of the genomes of simian sarcoma virus and its associated helper virus. NLM AIDSLINE Important note: Information in this article was accurate in 1981. The state of the art may have changed since the publication date.

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Molecular cloning and comparative analyses of the genomes of simian sarcoma virus and its associated helper virus.

Proc Natl Acad Sci U S A. 1981 Jun;78(6):3373-7. Unique Identifier : AIDSLINE MED/81273062
Gelmann EP; Wong-Staal F; Kramer RA; Gallo RC

Abstract: Closed circular viral DNA of simian sarcoma virus (SSV) and simian sarcoma-associated virus (SSAV) obtained from acutely infected dog cells was purified on preparative agarose gels, cleaved with EcoRI, and cloned in the phage lambda vector Charon 21A. The cloned 9-kilobase SSAV genome (B11) has the same restriction map as the bulk of the unintegrated linear SSAV DNA intermediate. Heteroduplex analysis between an SSV clone (lambda-C60) and an SSAV clone (lambda-B11) showed two substitution loops and one deletion loop. By using detailed restriction enzyme mapping and electron microscopic analysis, we showed that one of the substitution loops corresponds to an inversion of one of the two long terminal repeat units and adjacent cellular sequences in C60. The other substitution loop mapped close to the 3' long terminal repeat. At least part of this region was shown to contain SSV-specific sequences not shared by SSAV. The 1.9-kilobase deletion mapped at 3.5-5.5 kilobases of the linear SSAV genome, corresponding to most, if not all, of the pol gene.
Keywords: Chromosome Mapping Cloning, Molecular/METHODS DNA Restriction Enzymes/METABOLISM DNA, Viral/*GENETICS *Genes, Viral Helper Viruses/*GENETICS Inversion (Genetics) Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid Retroviridae/*GENETICS Sarcoma Viruses, Simian/*GENETICS JOURNAL ARTICLEKWDchromosomemappingcloning,molecular/methodsdnarestrictionenzymes/metabolismdna,viral/KWDgeneticsKWDgenes,viralhelperviruses/KWDgeneticsinversion(genetics)nucleicacidhybridizationrepetitivesequences,nucleicacidretroviridae/KWDgeneticssarcomaviruses,simian/KWDgeneticsjournalarticle
811230
M81C0001

Copyright © 1981 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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