Isolation and partial characterization of the reverse transcriptases of Rauscher murine leukemia virus, simian sarcoma virus and RD-114 virus. NLM AIDSLINE Important note: Information in this article was accurate in 1981. The state of the art may have changed since the publication date.

Click here to return to AIDSLINE main menu
DonateNow
Print this Article


Isolation and partial characterization of the reverse transcriptases of Rauscher murine leukemia virus, simian sarcoma virus and RD-114 virus.

Biochim Biophys Acta. 1980 Oct 17;609(3):483-91. Unique Identifier : AIDSLINE MED/81063235
van Muijen GN; Warnaar SO

Abstract: Reverse transcriptases were purified from Rauscher murine leukemia virus, Simian sarcoma virus and RD-114 virus by affinity chromatography using poly(rC)-Sepharose. Molecular weight determinations of the viral enzymes gave values of 80,000 for all three enzyme preparations. Two-dimensional tryptic peptide maps of the purified enzymes showed only a few spots in similar positions.
Keywords: Animal Cats/MICROBIOLOGY Chromatography, Affinity Comparative Study Molecular Weight Rauscher Virus/*ENZYMOLOGY Retroviridae/*ENZYMOLOGY Reverse Transcriptase/*ISOLATION & PURIF Sarcoma Viruses, Simian/*ENZYMOLOGY Species Specificity Support, Non-U.S. Gov't JOURNAL ARTICLEKWDanimalcats/microbiologychromatography,affinitycomparativestudymolecularweightrauschervirus/KWDenzymologyretroviridae/KWDenzymologyreversetranscriptase/KWDisolation&purifsarcomaviruses,simian/KWDenzymologyspeciesspecificitysupport,non-uKWDsKWDgov'tjournalarticle
810430
M8140001

Copyright © 1981 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1981. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1981. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .