Isolation of a virus closely related to gibbon ape leukaemia virus from cells infected with virus (HL-23V) released by human leukaemic cells. NLM AIDSLINE Important note: Information in this article was accurate in 1980. The state of the art may have changed since the publication date.

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Isolation of a virus closely related to gibbon ape leukaemia virus from cells infected with virus (HL-23V) released by human leukaemic cells.

J Gen Virol. 1980 May;48(1):111-21. Unique Identifier : AIDSLINE MED/80205996
Bergholz CM; Wolfe LG; Schulz GA; Deinhardt F; Miller NR; Reitz MS

Abstract: Canine thymus cells infected with virus (HL-23V) produced by human acute myelogenous leukaemia cells in culture were shown in previous reports to produce transforming and non-transforming type C virus similar or identical to the simian sarcoma virus complex SSV(SSAV) and to induce tumours in marmoset monkeys (Bergholz et al. 1977a). In these earlier studies the appearance of breakthrough foci at low dilutions of antiserum in neutralization tests with high-titred anti-SSV(SSAV) serum suggested the presence of another virus, distinct from SSV-(SSAV). We now report the isolation of this component and, by comparative neutralization analysis, demonstrate that it is most closely related to gibbon ape leukaemia virus (GALV). It is distinguished from SSV(SSAV) by kinetics of neutralization and molecular hybridization experiments. This component was readily cloned both from virus produced by HL-23V chronically-infected canine thymus cells established by Teich et al. (1975) when HL-23V was first isolated and from virus produced by HL-23V-induced marmoset tumour cells in culture. The presence of this component in the original leukaemic cell cultures is discussed.
Keywords: Animal Antigens, Viral/ANALYSIS Cell Line Cell Transformation, Viral Human Hylobates/MICROBIOLOGY Leukemia, Myelocytic, Acute/*MICROBIOLOGY Neutralization Tests Nucleic Acid Hybridization Retroviridae/ANALYSIS/GROWTH & DEVELOPMENT/*ISOLATION & PURIF Sarcoma Viruses, Simian/ANALYSIS Satellite Viruses/ANALYSIS JOURNAL ARTICLEKWDanimalantigens,viral/analysiscelllinecelltransformation,viralhumanhylobates/microbiologyleukemia,myelocytic,acute/KWDmicrobiologyneutralizationtestsnucleicacidhybridizationretroviridae/analysis/growth&development/KWDisolation&purifsarcomaviruses,simian/analysissatelliteviruses/analysisjournalarticle
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M80A0002

Copyright © 1980 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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